改

All is well. Try not to panic. Things will be EVEN BETTER than fine; the angels WANT you to be Baker-acted -- no wait, wrong conversation...

The Truth is OUT There ... and if it's not; just go ahead and make things up. Because, really, in The End (Apocalypse Now Remix), everything is always all-good. You only need to open your ears to see the truth, and understand that Saint Cobain died for our sins, Tupac was killed because he cared TOO MUCH, and Jesus so loved LSD that he gave the world bisexual women in thigh-high leather boots.

Complete Documented Proof is available from the Scientist in Outer Space, upon request.

Liff on the farm... (Post~Script)

There's a general sense of weirdness—wars which last for ever and are going nowhere, and policies which are nothing but rhetoric... they bear no relation to the facts....
—Gethsemane

煩悩即菩提 [Rip, Blog]

I sometimes wonder if that is what Krishna meant -
Among other things - or one way of putting the same
.....thing:
That the future is a faded song, a Royal rose or a lavender
.....spray
Of wistful regret for those who are not yet here to regret,
Pressed between yellow leaves of a book that has never been
.....opened.
And the way up is the way down, the way forward is the
.....way back.
- T. S. Eliot

From Budapest, with love.

#FNLAMEN:♦t5jiQeEuHY

It's All in Your Head,
You Don't Know Us,
You Can't See Us,
It's Just a Game,
We're Equals,
Expect Us...

'An, Hero'

Through a Looking Glass

Value = TTAATTA56(?)T

Input@Google -> TTAATTA 56 T

Return = Result 53;

{
Abstract

Background

GATA-2 is a transcription factor required for hematopoietic stem cell survival as well as for neuronal development in vertebrates. It has been shown that specific expression of GATA-2 in blood progenitor cells requires distal cis-acting regulatory elements. Identification and characterization of these elements should help elucidating transcription regulatory mechanisms of GATA-2 expression in hematopoietic lineage.

Results

By pair-wise alignments of the zebrafish genomic sequences flanking GATA-2 to orthologous regions of fugu, mouse, rat and human genomes, we identified three highly conserved non-coding sequences in the genomic region flanking GATA-2, two upstream of GATA-2 and another downstream. Using both transposon and bacterial artificial chromosome mediated germline transgenic zebrafish analyses, one of the sequences was established as necessary and sufficient to direct hematopoietic GFP expression in a manner that recapitulates that of GATA-2. In addition, we demonstrated that this element has enhancer activity in mammalian myeloid leukemia cell lines, thus validating its functional conservation among vertebrate species. Further analysis of potential transcription factor binding sites suggested that integrity of the putative HOXA3 and LMO2 sites is required for regulating GATA-2/GFP hematopoietic expression.

Conclusion

Regulation of GATA-2 expression in hematopoietic cells is likely conserved among vertebrate animals. The integrated approach described here, drawing on embryological, transgenesis and computational methods, should be generally applicable to analyze tissue-specific gene regulation involving distal DNA cis-acting elements."
}

Gakked From:
www.biomedcentral.com/content/ download/xml/1471-213x-7-97.xml - 113k -